abstract：:In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. ...

journal_title：Journal of microbiological methods

authors： Huber CA,Pflüger V,Reed S,Cottrell K,Sidjabat HE,Ranasinghe A,Zowawi HM,Harris P,Paterson DL

更新日期：2019-09-01 00:00:00

abstract：:RNA-based molecular technique (RT-qPCR) is a promising method for microcystin monitoring in lakes and reservoirs, but great lability of RNA in cyanobacterial samples limits its application. To date, no studies have investigated how to effectively preserve RNA in cyanobacterial samples. In this study, four different tr...

journal_title：Journal of microbiological methods

authors： Li X,Qiu D,Chen S,Li J,Luo C,Hu D,Li J,Zhu J,Chen H,Li S,Yu X

更新日期：2019-09-01 00:00:00

abstract：:In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were f...

journal_title：Journal of microbiological methods

authors： Kasap EN,Doğan Ü,Çoğun F,Yıldırım E,Boyacı İH,Çetin D,Suludere Z,Tamer U,Ertaş N

更新日期：2019-09-01 00:00:00

abstract：:The U.S. Environmental Protection Agency Alternative Test Procedure protocol outlines a method to produce chlorine-stressed bacteria for water quality testing. Achieving consistent results is challenging due effluent variability. We describe a starting point for generating chlorine-stressed samples from secondary effl...

journal_title：Journal of microbiological methods

authors： Hutchison JR,Widder MW,Brooks SM,Brennan LM,Souris L,Divito VT,van der Schalie WH,Ozanich RM

更新日期：2019-08-01 00:00:00

abstract：:The basic requirement of human beings is better health but the serious health effects and numerous infections caused by rapid growth of harmful pathogens resulting in a large number of deaths and is a significant challenge to modern science. Microbes infecting humans can be stopped in two ways: disinfectants and antim...

journal_title：Journal of microbiological methods

authors： Nagaraja A,Jalageri MD,Puttaiahgowda YM,Raghava Reddy K,Raghu AV

更新日期：2019-08-01 00:00:00

abstract：:Traditional antibiotic susceptibility testing methods take several days to confirm and start disease treatment. The lack of new antibiotics or drugs warrants a need for optimization of current diagnostic tools for immediate antibiotic susceptibility testing. We present a rapid screening method to evaluate the response...

journal_title：Journal of microbiological methods

authors： Mishra P,Singh D,Mishra KP,Kaur G,Dhull N,Tomar M,Gupta V,Kumar B,Ganju L

更新日期：2019-07-01 00:00:00

abstract：:A simple generic toxicity method (test) is proposed using baker's yeast to mediate the reduction of the colourless triphenyltetrazolium chloride (TTC) to red, 1,3,5-triphenyl formazan, which can be extracted by dimethyl sulfoxide (DMSO), enabling the identification of reducible toxic compounds (e.g. cadmium, fipronil)...

journal_title：Journal of microbiological methods

authors： Gomes LH,Duarte KMR,Kamogawa MY,Ferrarezi JA,Andrino FG,Tavares ACL,da Silva APM,Laguna SE,de Lira SP

更新日期：2019-06-01 00:00:00

abstract：:The paper and pulp industry (PPI) produces high quantities of solid and liquid discharge and is regarded as the most polluting industry in the world causing adverse effects to environments and human beings. Hence changes in the way PPI sludge and waste materials are treated is urgently required. Nearly, 10 million ton...

journal_title：Journal of microbiological methods

authors： Chakraborty D,Shelvapulle S,Reddy KR,Kulkarni RV,Puttaiahgowda YM,Naveen S,Raghu AV

更新日期：2019-05-01 00:00:00

abstract：:Bacterial identification methods used in routine identification of pathogens in medical microbiology include a combination approach of biochemical tests, mass spectrometry or molecular biology techniques. Extensive publicly-available databases of DNA sequence data from pathogenic bacteria have been amassed in recent y...

journal_title：Journal of microbiological methods

authors： Talamantes-Becerra B,Carling J,Kennedy K,Gahan ME,Georges A

更新日期：2019-05-01 00:00:00

abstract：:Laser desorption-time of flight (LD-TOF) mass spectrometry-based detection of hemozoin was assessed for its performance characteristics as a rapid screening test for malaria. In spite of good specificity of >95%, poor sensitivity of 80.2% for microscopically positive samples makes the easy-to-apply and rapid approach ...

journal_title：Journal of microbiological methods

authors： Christner M,Frickmann H,Klupp E,Rohde H,Kono M,Tannich E,Poppert S

更新日期：2019-05-01 00:00:00

abstract：:Synthesis and application of reliable nanoscale materials is a progressive domain and the limelight of modern nanotechnology. Conventional physicochemical approaches for the synthesis of metal nanoparticles have become obsolete owing to costly and hazardous materials. There is a need to explore alternative, cost-effec...

journal_title：Journal of microbiological methods

authors： Ali J,Ali N,Wang L,Waseem H,Pan G

更新日期：2019-04-01 00:00:00

abstract：:Bacterial biofilms are populations of bacteria within a self-produced adherent extracellular matrix that are notoriously resistant to treatment. Existing methods for biofilm quantification are often limited in their dynamic range of detection (signal-to-background), throughput, and require modifications to the protoco...

journal_title：Journal of microbiological methods

authors： Rajamani S,Sandy R,Kota K,Lundh L,Gomba G,Recabo K,Duplantier A,Panchal RG

更新日期：2019-04-01 00:00:00

abstract：:In recent years, the rapid advances of culture-independent methods and new molecular tools have revolutionized our understanding of microbial biodiversity and ecological functions. DNA extraction from microbial communities is a critical step in this process and several methods have been proposed and used, but the infl...

journal_title：Journal of microbiological methods

authors： Mateus-Barros E,Meneghine AK,Bagatini IL,Fernandes CC,Kishi LT,Vieira AAH,Sarmento H

更新日期：2019-04-01 00:00:00

abstract：:Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax citrulli, is a major threat to commercial watermelon and melon production worldwide. At present, there are at least two genetically distinct sub-populations (group I and II) of A. citrulli that differ in host preference among cucurbit species and copper s...

journal_title：Journal of microbiological methods

authors： Yang YW,Zhao M,Zhang LQ,Qiao P,Bai X,Zhang XX,Walcott RR,Guan W,Zhao TC

更新日期：2019-03-01 00:00:00

abstract：:Vibrio cholerae strains producing cholera toxin (CT) and toxin co-regulated pilus (TCP) and belonging to O1 and O139 serogroups are responsible for cholera. However, non-CT producing V. cholerae from non-O1/non-O139 serogroups have been increasingly isolated from diarrheal stools and extra-intestinal infections. In th...

journal_title：Journal of microbiological methods

authors： Awasthi SP,Chowdhury N,Neogi SB,Hinenoya A,Hatanaka N,Chowdhury G,Ramamurthy T,Yamasaki S

更新日期：2019-02-01 00:00:00

abstract：:Vibrio parahaemolyticus is one of the important foodborne pathogens is of public health concern due to the emergence of pandemic strains causing disease outbreaks worldwide. We evaluated the DNA based colony hybridization technique for the detection and enumeration of total and pathogenic V. parahaemolyticus from the ...

journal_title：Journal of microbiological methods

authors： Anupama KP,Deeksha K,Deeksha A,Karunasagar I,Karunasagar I,Maiti B

更新日期：2019-02-01 00:00:00

abstract：:Freeze-thaw DNA extraction methods and PCR primers were compared to optimize detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in different matrices. Increasing FT cycles did not increase parasite DNA detection, and primers targeting the 18S ssrRNA gene yielded the most sensitive detection of C. parvum...

journal_title：Journal of microbiological methods

authors： Manore AJW,Harper SL,Aguilar B,Weese JS,Shapiro K

更新日期：2019-01-01 00:00:00

abstract：:Plant-associated bacteria produce quorum sensing (QS) signals for community (biofilm) formation and functioning in the rhizosphere. The QS-positive biofilm-forming rhizobacteria that excel benefits to the plants are now gaining increased importance for agricultural use due to their high competitiveness. However, there...

journal_title：Journal of microbiological methods

authors： Begum JF,Tamilarasi M,Pushpakanth P,Balachandar D

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:In a two-stage exchange protocol for prosthetic joint infection (PJI), bacteria surviving over the antibiotic-loaded cement spacers may cause the persistence of infection with renewed clinical symptoms following the surgery. Culture after sonication of removed prosthesis is more sensitive than conventional p...

journal_title：Journal of microbiological methods

authors： Sambri A,Maso A,Storni E,Donati ME,Pederzoli A,Dallari D,Bianchi G,Donati DM

更新日期：2019-01-01 00:00:00

abstract：:Nosocomial infections caused by Stenotrophomonas maltophilia resistant to SXT are increasingly reported worldwide. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of the ssrA and sul1, sul2 and sul3 genes was first established. The assays were performed on a Roch...

journal_title：Journal of microbiological methods

authors： Li S,Peng Y,Rui Y

更新日期：2019-01-01 00:00:00

abstract：:Xylella fastidiosa is an insect-transmitted bacterial plant pathogen which causes a variety of economically important diseases worldwide. Molecular identification of X. fastidiosa is used for quarantine screening, surveillance, and research applications; many of which require subspecies level differentiation of pathog...

journal_title：Journal of microbiological methods

authors： Burbank LP,Ortega BC

更新日期：2018-12-01 00:00:00

abstract：:The susceptibility protocol established in this study takes into account that complex media are capable to buffer H2O2 which otherwise may adulterate test results. Furthermore, we demonstrate that clinical isolates of Staphylococcus aureus showed a higher resistance against H2O2 than Streptococcus pyogenes after 30 mi...

journal_title：Journal of microbiological methods

authors： Menschner L,Falke U,Töpfner N,Berner R

更新日期：2018-12-01 00:00:00

abstract：:We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its ...

journal_title：Journal of microbiological methods

authors： Aono A,Chikamatsu K,Yamada H,Igarashi Y,Murase Y,Takaki A,Mitarai S

更新日期：2018-11-01 00:00:00

abstract：:Characterization of airborne bacterial cells requires efficient collection, concentration, and analysis techniques, particularly to overcome the challenge of their dilute nature in outdoor environments. This study aims to establish a rapid and reliable approach for quantification of bacteria in air samples. To do this...

journal_title：Journal of microbiological methods

authors： Jang J,Hendriksen NB,Jakobsen HH,Gosewinkel U

更新日期：2018-11-01 00:00:00

abstract：:Metagenomic sequencing of bacterial samples has become the gold standard for profiling microbial populations, but 16S rRNA profiling remains widely used due to advantages in sample throughput, cost, and sensitivity even though the approach is hampered by primer bias and lack of specificity. We hypothesized that a hybr...

journal_title：Journal of microbiological methods

authors： Schriefer AE,Cliften PF,Hibberd MC,Sawyer C,Brown-Kennerly V,Burcea L,Klotz E,Crosby SD,Gordon JI,Head RD

更新日期：2018-11-01 00:00:00

abstract：:In the current study, a duplex PCR-ELISA method was developed targeting the specific genes, invA of Salmonella spp. and rfbE of Escherichia coli O157: H7, to detect one or both bacteria in food. In brief, PCR product amplified by PCR primer labeled with digoxin at the 5'-end and a probe labeled with biotin at the 3'-e...

journal_title：Journal of microbiological methods

authors： Hu J,Huang R,Wang Y,Wei X,Wang Z,Geng Y,Jing J,Gao H,Sun X,Dong C,Jiang C

更新日期：2018-11-01 00:00:00

abstract：:We monitored Chlamydia trachomatis growth in HeLa cells cultured with either DMEM or RPMI medium containing 10% FCS under 2% or 21% O2 conditions for 2 days. Bacterial numbers, host cell numbers, and fibrosis-related gene expression in the host cells were estimated by an inclusion forming unit assay, a cell counting a...

journal_title：Journal of microbiological methods

authors： Sakai K,Matsuo J,Watanabe T,Okubo T,Nakamura S,Yamaguchi H

更新日期：2018-10-01 00:00:00

abstract：:Counting colonies is usually used in microbiological analysis to assess if samples meet microbiological criteria. Although manual counting remains gold standard, the process is subjective, tedious, and time-consuming. Some developed automatic counting methods could save labors and time, but their results are easily af...

journal_title：Journal of microbiological methods

authors： Zhu G,Yan B,Xing M,Tian C

更新日期：2018-10-01 00:00:00

abstract：:The aim of this study was to (i) design, develop and validate a practical and physiologically relevant reconstituted in vitro oral mucosa tissue model and (ii) to assess its applicability in in vitro host-pathogen interactions with C. albicans and S. aureus. Co-culture organotypic constructions were created by incorpo...

journal_title：Journal of microbiological methods

authors： de Carvalho Dias K,de Sousa DL,Barbugli PA,Cerri PS,Salih VM,Vergani CE

更新日期：2018-09-01 00:00:00

abstract：:Confocal microscopy-based monitoring of pH in biofilms is gaining increasing interest, as it allows for a quick assessment of horizontal pH gradients without mechanically perturbing the biofilm. Ratiometric monitoring of pH with the fluorescent dye C-SNARF-4 has been used to reliably map extracellular pH in the basal ...

journal_title：Journal of microbiological methods

authors： Schlafer S,Baelum V,Dige I

更新日期：2018-09-01 00:00:00

abstract：:Lactobacillus animalis NP51 is a direct-fed microbial strain (DFM) extensively used as a pre-harvest food safety mitigation in feedlot cattle due to its antagonistic effects against human foodborne pathogens such as Salmonella and Escherichia coli O157:H7. NP51 not only promotes overall gut health but interferes with ...

journal_title：Journal of microbiological methods

authors： Ayala DI,Chen JC,Bugarel M,Loneragan GH,den Bakker HC,Kottapalli KR,Brashears MM,Nightingale KK

更新日期：2018-06-01 00:00:00

abstract：:Quantitative real-time reverse transcriptase PCR (qRT-PCR) has become the method choice for quantification of gene expression changes, however, the accuracy of the method depends on the stability of reference genes. Ralstonia pseudosolanacearum (R. pseudosolanacearum) is an important plant pathogen, infecting >450 pla...

journal_title：Journal of microbiological methods

authors： Yang L,Chen J,Liu Y,Zhang S,Li S,Ding W

更新日期：2018-05-01 00:00:00

abstract：:Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the ...

journal_title：Journal of microbiological methods

authors： Stumpp NS,Konze SA,Gerardy-Schahn R,Stiesch M,Buettner FFR

更新日期：2018-05-01 00:00:00

abstract：:Bacillus subtilis pheS was genetically modified to obtain a counter-selection marker with high selection efficiency in Bacillus amyloliquefaciens. The application of the new replication-thermosensitive integrative vector pNZTM1, containing this marker, pheSBsT255S/A309G, with a two-step replacement recombination proce...

journal_title：Journal of microbiological methods

authors： Kharchenko MS,Teslya PN,Babaeva MN,Zakataeva NP

更新日期：2018-05-01 00:00:00

abstract：:Systems that automate extraction of nucleic acid from cells or viruses in complex clinical matrices have tremendous value even in the absence of an integrated downstream detector. We describe our bench-top automated workstation that integrates our previously-reported extraction method - TruTip - with our newly-develop...

journal_title：Journal of microbiological methods

authors： Thakore N,Garber S,Bueno A,Qu P,Norville R,Villanueva M,Chandler DP,Holmberg R,Cooney CG

更新日期：2018-05-01 00:00:00

abstract：:Culture-independent molecular techniques and advances in next generation sequencing (NGS) technologies make large-scale epidemiological studies on microbiota feasible. A challenge using NGS is to obtain high reproducibility and repeatability, which is mostly attained through robust amplification. We aimed to assess th...

journal_title：Journal of microbiological methods

authors： Raju SC,Lagström S,Ellonen P,de Vos WM,Eriksson JG,Weiderpass E,Rounge TB

更新日期：2018-04-01 00:00:00

abstract：:The mesophilic fungus Eupenicillium parvum 4-14 is an important producer of thermotolerant hemicellulolytic and cellulolytic enzymes. The aim of this study was to establish a method for genetic manipulation of the fungus by Agrobacterium tumefaciens. The promotor PgpdA of a glyceraldehyde-3-phosphate dehydrogenase gen...

journal_title：Journal of microbiological methods

authors： Long L,Lin Q,Shi Y,Wang J,Ding S

更新日期：2018-03-01 00:00:00

abstract：:Chemotactic behaviour is an important part of the lifestyle of motile bacteria and enables cells to respond to various environmental stimuli. The Hard Agar Plug (HAP) method is used to study the chemotactic behaviour of bacteria, including the fastidious microaerophile Campylobacter jejuni, an intestinal pathogen of h...

journal_title：Journal of microbiological methods

authors： Elgamoudi BA,Ketley JM,Korolik V

更新日期：2018-03-01 00:00:00

abstract：:Rapid pathogen identification (ID) and antimicrobial susceptibility testing (AST) in bacteremia cases or sepsis could improve patient prognosis. Thus, it is important to provide timely reports, which make it possible for clinicians to set up appropriate antibiotic therapy during the early stages of bloodstream infecti...

journal_title：Journal of microbiological methods

authors： Sakarikou C,Altieri A,Bossa MC,Minelli S,Dolfa C,Piperno M,Favalli C

更新日期：2018-03-01 00:00:00

abstract：:The ELISA is recognized as an efficient diagnostic tool for antibody detection, but there is no standard ELISA assay for detection of antibodies against hemorrhagic septicemia (HS) in cattle. The present study reports on an indirect ELISA assay for antibody detection of HS in dairy cows, and evaluates the sensitivity ...

journal_title：Journal of microbiological methods

authors： Tankaew P,Srisawat W,Singhla T,Tragoolpua K,Kataoka Y,Sawada T,Sthitmatee N

更新日期：2018-02-01 00:00:00